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KMID : 1189420200330010001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2020 Volume.33 No. 1 p.1 ~ p.11
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Anti-inflammatory and Anti-oxidant Studies of Osung-tang Extracts in LPS-Induced RAW 264.7 Cells
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Kim So-Jeong
Kim Tae-Jun
Kim Ee-Hwa
Kim Yong-Min
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Abstract
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Objectives : The purpose of this study was to investigate the anti-inflammatory and anti-oxidative effects of Osung?tang(OST) extract.
Methods : MTT assay was performed to confirm the survival rate of RAW 264.7 cells treated with OST extract(50-400㎍/㎖) and the production of NO from LPS-induced RAW 264.7 cells was confirmed. The mRNA expression of iNOS, COX-2, and NF-κB were measured by real-time PCR. The protein expression of iNOS, COX-2 and p-IκB were measured by western blot and the anti-oxidant activity of OST extract(50-400㎍/㎖) was investigated by measuring DPPH scavenging activity.
Results : OST extract showed a cell survival rate of 90% or more at 50-400㎍/㎖. The NO production was inhibited in a dose-dependent manner in RAW 264.7 cells treated with LPS on OST treated group. mRNA expression levels of iNOS, COX-2 and NF-κB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). Protein expression levels of iNOS, COX-2 and p-IκB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). It was found that OST has a high free-radical scavenging ability.
Conclusions : These results suggest that OST extract can be used as a treatment for various skin diseases by demonstrating its anti-inflammatory and anti-oxidant effects.
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KEYWORD
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Anti-inflammation, Anti-oxidant, COX-2, iNOS, NF-κB, Osung-tang(OST)
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